Preheat the oven to 350°F. Lightly grease a 9x5-inch loaf pan with cooking spray.
In a large bowl, combine the eggs, Panko breadcrumbs, onion, garlic, ketchup, Worcestershire sauce, dried thyme, garlic powder, salt, and pepper. Stir with a fork until well combined.
Add the ground beef to the bowl and mix everything together with your hands until well combined, being careful not to over-mix.
Shape the mixture into a loaf and place it in the greased loaf pan.
For the meatloaf glaze, combine ½ cup ketchup, brown sugar, and apple cider vinegar in a small bowl. Whisk until smooth.
Bake the meatloaf for 45 minutes, then spread the glaze over the top and continue baking for 35-45 more minutes, or until the meatloaf reaches 160°F in the center when tested with an instant-read thermometer.
Let the meatloaf rest for 10 minutes before slicing and serving.